Author/Authors :
Chen, Zhibao College of Life Science and Technology - Heilongjiang Bayi Agricultural University - Daqing, Heilongjiang 163319, China , Li, Xinran College of Life Science and Technology - Heilongjiang Bayi Agricultural University - Daqing, Heilongjiang 163319, China , Wu, Xiuping Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China , Wang, Wei Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China , Wang, Wendong Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China , Xin, Mingxun Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China , Shen, Fengge Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China , Liu, Lihui Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China , Liang, Junchao Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China , Li, Lei Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China , Yu, Lu Key Laboratory of Zoonosis Research - Ministry of Education - Institute of Zoonosis - College of Animal Science and Veterinary Medicine - Jilin University - Changchun, China
Abstract :
The aim of this investigation was to assess the in-vitro interaction of two antifungal
agents, econazole-nitrate and chelerythrine, against ten fluconazole-resistant clinical isolates and one ATCC type strain 10231 of Candida albicans. The checkerboard microdilution method was performed according to the recommendations of the National Committee for Clinical Laboratory Standards, and the results were determined by visual examination. The interaction intensity was tested in all isolates using the fractional inhibitory concentration index (FICI). These experiments showed synergism between econazole-nitrate and chelerythrine in antifungal activity against C. albicans, and no antagonistic activity was observed in any of the strains tested. Moreover, time-kill curves were performed with selected strains to confirm the positive interactions. The similarity between the results of the FICI values and the timekill curves revealed that chelerythrine greatly enhances the antifungal effects of econazolenitrate against isolates of C. albicans. This synergistic effect may markedly reduce the dose of
econazole-nitrate required to treat candidiasis, thereby decreasing the econazole-nitrate toxic side effects. This novel synergism might provide a potential combination treatment against fungal infections.
Keywords :
Candida albicans , Synergism , Econazole-nitrate , Chelerythrine
