Author/Authors :
Jalali Tehrani, Hora Depatment of Biology - Science and Research Branch Islamic Azad University Tehran , Parivar, Kazem Depatment of Biology - Science and Research Branch Islamic Azad University Tehran , Ai, Jafar Department of Tissue Engineering - Faculty of Advance Technology - University of Medical Sciences, Tehran , Kajbafzadeh, Abdolmohammad Paediatric Urology Research Center - Department of Urology - Children›s Hospital Medical Center - Tehran University of Medical Sciences,Tehran , Rahbarghazi, Reza Umbilical Cord Stem Cell Research Center - Tabriz University of Medical Sciences , Hashemi, Mehrdad Department of Medical Genetics - Tehran Medical Sciences Branch - Islamic Azad University Tehran , Sadeghizadeh, Majid Department of Genetics - Faculty of Biological Sciences - Tarbiat Modares University, Tehran
Abstract :
Human endometrium contains mesenchymal stem cells (eMSC) which have the ability
to differentiate into three cell lineages and the potential in therapeutic applications. We hypothesize that using environmental induction in culture media such as dexamethasone, human recombinant insulin and human epidermal growth factor (hEGF) can differentiate endometrial stem cells into myoblast. These agents have a broad range of effects in myoblast differentiation in-vitro. We used immunohystochemistry analysis and RT –PCR to evaluate the presence of skeletal muscle - specific proteins some of which are expressed in the early stage of
differentiation including myoD and Desmin which expressed at later stages of differentiation. In conclusion eMSC can differentiate in culture media which contains above mentioned factors and use for therapeutic purpose in muscular degenerative disease.
Keywords :
Dexamethasone , Endometrial stem cell , Myogenic potential , Differentiation
