Author/Authors :
Sales, Valterlúcio dos Santos Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazil , Monteiro, Alefe Brito Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Delmondes, Gyllyandeson de Araujo Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Nascimentoa, Emmily Petícia do Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Figueiredo, Francisco Rodolpho Sobreira Dantas Nóbrega de Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Rodrigues, Cristina Kelly de Souza Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Lacerda, Josefa Fernanda Evangelista de Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Fernandes, Cícera Norma Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Barbosa, Maysa de Oliveira Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Brasil, Adamo Xenofonte Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Tintino, Saulo Relison Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Gomezb, Maria Celeste Vega Center for the Development of Scientific Research - Foundation Moisés Bertoni/Laboratories Diaz Gill, Asunción-Paraguay , Coronel, Cathia Center for the Development of Scientific Research - Foundation Moisés Bertoni/Laboratories Diaz Gill, Asunción-Paraguay , Coutinho, Henrique Douglas Melo Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Costaa, José Galberto Martins da Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Felipe, Cícero Francisco Bezerra Department of Molecular Biology - Federal University of Paraíba, Paraíba, PB, Brazil , Menezes, Irwin Rose Alencar de Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi , Kerntopfa, Marta Regina Department of Biological Chemistry - Regional University of Cariri, Crato, CE, Brazi
Abstract :
With the increase of neglected diseases such as leishmaniasis and Chagas disease, there was
a need for the search for new therapeutic alternatives that reduce the harm caused by medicine
available for treatment. Thus, this study was performed to investigate the antiparasitic activity
of the essential oil from the fruits of Piper tuberculatum Jacq, against lines of Leishmania
braziliensis (MHOM/CO/88/UA301), Leishmania infantum (MHOM/ES/92/BCN83) and
Trypanosoma cruzi (LC-B5 clone). Before running protocols, an analysis of the chemical
composition of essential oil was conducted, which presented monoterpenes and sesquiterpenes.
As major constituents, β-pinene and α-pinene were identified. Regarding to antiparasitic activity,
the essential oil had an EC50 values of 133.97 μg/mL and 143.59 μg/mL against variations
promastigotes of L. infantum and L. braziliensis, respectively. As for trypanocidal activity, the
oil showed EC50 value of 140.31 μg/mL against epimastigote form of T. cruzi. Moreover, it
showed moderate cytotoxicity in fibroblasts with LC50 value of 204.71 μg/mL. The observed
effect may be related to the presence of terpenes contained in the essential oil, since it has its
antiparasitic activity proven in the literature.
