Author/Authors :
Shamsabadipour, Sara Department of Biology - Faculty of Science - University of Isfahan, Isfahan, I.R. Iran , Zarei, Mohammad Phytochemistry Research Center - Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran , Ghanadian, Mustafa Isfahan Pharmaceutical Sciences Research Center - Isfahan University of Medical Sciences, I.R. Iran , Ayatollahi, Abdulmajid Phytochemistry Research Center - Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran , Rahimnejad, Mohammad Reza Department of Biology - Faculty of Science - University of Isfahan, Isfahan, I.R. Iran , Saeedi, Hojjatollah Department of Biology - Faculty of Science - University of Isfahan, Isfahan, I.R. Iran , Aghaei, Mahmoud Biochemistry Department - Isfahan Faculty of Pharmacy and Pharmaceutical Sciences - Isfahan University of Medical Sciences, Isfahan, I.R. Iran
Abstract :
In this research, aerial parts of Euphorbia denticulata Lam were collected from Sanandaj
city in Kurdistan province at the West of Iran. It was extracted by maceration using acetone as
solvent. The isolation of compounds were carried out with repeated column chromatography
using silica gel and normal preparative HPLC using YMC-Pack-Sil column and hexane: ethyl
acetate as mobile phase. The structures of isolated compounds were elucidated by extensive
1 and 2D-NMR as well as HRESI-MS spectra and the cytotoxicity was done against DU-145
human prostate cancer cell line using standard MTT assay. It afforded a new 12-taraxastane
derivative and two known cycloartane triterpenes including: taraxast-12-ene-3β,20,21(α)-triol
(1), cycloartane-3β,25-diol (2), and cycloartane-3β,24,25-triol (3). They exhibited cytotoxic
effects, with IC50 values of 12.2 ± 2.9, 27.5 ± 4.9, and 18.3 ± 1.4 μM, respectively.
Keywords :
Cytotoxicity , Prostate Cancer , Taraxastane , Cycloartane , Triterpenes , Euphorbia denticulata
