Author/Authors :
Sahraroo, Amir Department of Horticulture - Faculty of Agriculture - University of Tehran, Karaj , Babalar, Mesbah Department of Horticulture - Faculty of Agriculture - University of Tehran, Karaj , Mirjalili, Mohammad Hossein Department of Agriculture - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University - G. C., Evin, Tehran , Fattahi Moghaddam, Mohammad Reza Department of Agriculture - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University - G. C., Evin, Tehran , Nejad Ebrahimi, Samad Department of Phytochemistry - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University - G. C., Evin, Tehran
Abstract :
In the present study, an efficient protocol has been developed for callus induction and
production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL-1) solely or in combination with cytokinins BAP and KIN (1.0, 2.0 and 5.0 mgL-1). B5 medium supplemented with 1.0 mgL-1 IBA plus 5.0 mgL-1 BAP and MS medium fortified with 2.0 mgL-1 IBA and 2.0 mgL-1 BAP were the most favorable media for callus formation with the highest induction rate (96%). Maximum growth index (2.89 and 2.63) and maximum callus biomass (2.34 and 2.33 g fresh weight) were obtained from the callus cultured on B5 medium supplemented with 1.0 mgL-1 IBA plus 5.0 mgL-1 BAP and MS medium fortified with 1.0 mgL-1 IBA plus 1.0 mgL-1 KIN, respectively. Determination and quantification of RA in cultured calli were performed
by HPLC UV/MS analysis. Calli induced from the plant and maintained on supplements
of IBA and BAP in the absence of light produced RA 7.5% based on dry weight (DW). No differentiation was observed in any callus during the course of this study.
Keywords :
Satureja sp , Lamiaceae , Callus culture , Rosmarinic acid , HPLC UV/MS
