Author/Authors :
Yin, Lei School of Pharmacy - Ningxia Medical University, Yinchuan 750004, China , Guan, Enshuang School of Pharmacy - Ningxia Medical University, Yinchuan 750004, China , Zhang, Yuanbin School of Pharmacy - Ningxia Medical University, Yinchuan 750004, China , Shu, Zhiheng School of Pharmacy - Ningxia Medical University, Yinchuan 750004, China , Wang, Bing School of Pharmacy - Ningxia Medical University, Yinchuan 750004, China , Wu, Xiuli School of Pharmacy - Ningxia Medical University, Yinchuan 750004, China , Chen, Jing School of Pharmacy - Ningxia Medical University, Yinchuan 750004, China , Liu, Jingxia School of traditional Chinese medicine - Ningxia Medical University, Yinchuan 750004, China , Fu, Xueyan School of Pharmacy - Ningxia Medical University, Yinchuan 750004, China , Sun, Weihong Depterment of General Hospital of Ningxia Medical University, Yinchuan 750004, China , Liu, Meifeng School of Chemistry and Chemical Engineering - Guangdong Provincial Key Lab for Green Chemical Product Technology - South China University of Technology, Guangzhou 510640, China
Abstract :
Glycyrrhiza uralensis Fisch. (G. uralensis) is one of the most widely used herbal medicines.
This study was designed to enrich total flavonoids (TFF) from G. uralensis. The chemical
profile of TFF was identified by HPLC and colorimetric assay. The TFF mainly contained
liquiritin apioside, liquiritin, isoliquiritin apioside, liquiritigenin and isoliquiritigenin without
glycyrrhizic acid. To study the anti-inflammatory activity of TFF, the DMB-induced ear
vasodilatation assay and carrageenan-induced rat paw edema model have been utilized.
Treatment with TFF showed significant anti-inflammatory activities in the two models. The
two in-vivo edema assays demonstrated that the TFF possesses significant dose-dependent antiinflammatory
activity, similar to that of indomethacin at a dose of 500 mg/kg. In rat paws with
carrageenan, treatment with TFF (500 and 250 mg/kg) markedly inhibited the expression of
IL-1β and iNOS. TFF at all doses noticeably decreased levels of NO and MDA at the site of
inflammation, while only i.g. TFF at a dose of 500 mg/kg significantly decreased TNF-α levels
in the carrageenan-injected paws. In addition, an increase in SOD activity was induced by TFF
at all doses. These results revealed that TFF exhibited significant anti-inflammatory activity in
acute inflammatory models.
Keywords :
ELISA , Total flavonoids , Glycyrrhiza uralensis Fisch , Anti-inflammatory , HPLC
