Author/Authors :
Peng, Yong Department of Biomedical Engineering - College of Electrical Engineering - Yanshan University - Qinhuangdao - 066004, China - Department of Biological Engineering - College of Environment - Chemical Engineering - Yanshan University - Qinhuangdao, 066004, China , Fu, Zhan-zhao The First Hospital of Qinhuangdao Town - Qinhuangdao, 066100, China , Guo, Cong-Shan Department of Biomedical Engineering - College of Electrical Engineering - Yanshan University - Qinhuangdao, 066004, China , Zhang, Yan-Xia Department of Biomedical Engineering - College of Electrical Engineering - Yanshan University - Qinhuangdao, 066004, China , Di, Ya The First Hospital of Qinhuangdao Town - Qinhuangdao, 066100, China , Jiang, Bin Department of Biomedical Engineering - College of Electrical Engineering - Yanshan University - Qinhuangdao, 066004, China , Li, Qing-Wang Department of Biological Engineering - College of Environment - Chemical Engineering - Yanshan University - Qinhuangdao - 066004, China - Department of Animal Science - Northwest Agriculture and Forestry University - Yangling, 712100, China
Abstract :
The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer HeLa cells. The inhibitory effect of baicalin on the growth of HeLa cells was measured by MTT assay, and cell proliferation and migration was analyzed by cell scratch assay. Morphological changes of apoptotic cells were viewed by the light microscope and electron microscope, and cell growth arrest was confirmed by flow cytometry. Moreover, Western blot was used for investigating the expression of apoptosis related proteins; spectrophotometry was used to examine Caspase-3 activation. Our results showed that baicalin could inhibit the proliferation of HeLa Cells via induction of apoptosis in a time and dosedependent
manner (P<0.01). Apoptotic signaling induced by baicalin was characterized by
up-regulating Bax, Fas, FasL and Caspase-8 protein expression, and down-regulating of Bcl-2 protein expression. These results indicated that baicalin-induced apoptosis involved activation Caspase-3 in HeLa cells through the intracellular mitochondrial pathway and the surface death receptor pathway.
Keywords :
Baicalin , HeLa cells , Cell apoptosis , Mitochondrial pathway , Death receptor pathway , Caspase-3
