Title of article :
An Accessible and Pragmatic Experimental Model of Nonalcoholic Fatty Liver Disease
Author/Authors :
Emamat, Hadi Department of Clinical Nutrition and Dietetics - Faculty of Nutrition Sciences and Food Technology - National Nutrition and Food Technology Research Institute - Shahid Beheshti University of Medical Sciences , Noori, Maryam Department of Clinical Nutrition and Dietetics - Faculty of Nutrition Sciences and Food Technology - National Nutrition and Food Technology Research Institute - Shahid Beheshti University of Medical Sciences , Foroughi, Forough Department of Pathology - Taleghani Hospital - Faculty of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Rismanchi, Marjan Department of Clinical Nutrition and Dietetics - Faculty of Nutrition Sciences and Food Technology - National Nutrition and Food Technology Research Institute - Shahid Beheshti University of Medical Sciences , Eini–Zinab, Hassan Department of Community Nutrition - Faculty of Nutrition Sciences and Food Technology - National Nutrition and Food Technology Research Institute - Shahid Beheshti University of Medical Sciences, Tehran , Hekmatdoost, Azita Department of Clinical Nutrition and Dietetics - Faculty of Nutrition Sciences and Food Technology - National Nutrition and Food Technology Research Institute - Shahid Beheshti University of Medical Sciences
Abstract :
BACKGROUND There is no convenient cheap pragmatic experimental model for Nonalcoholic Fatty Liver Disease (NAFLD)/Nonalcoholic Steatohepatitis (NASH). Objective: Our objective was to create a pragmatic model of NAFLD/NASH. METHODS
Sprague-Dawley rats were fed a high-fat, high sugar homemade diet ad libitum for
seven weeks. The high-fat, high sugar diet included 59% of energy derived from fat,
30% from carbohydrates, and 11% from protein. Serum levels of fasting glucose, triglyceride, cholesterol, liver enzymes, insulin, and hepatic tumor necrosis factor-alpha (TNF-α) gene expression were determined. Hepatic histology was examined by H&E stain. RESULTS Rats fed the high-fat, high sugar diet developed hepatic steatosis, and a moderate inflammation, which was associated with increased serum levels of liver enzymes, glucose, insulin, triglyceride, cholesterol, and hepatic TNF-α gene expression. CONCLUSION This rat model resembles the key features of human NAFLD/NASH and provides a simple pragmatic experimental model for elucidating the disease prevention and treatment.
Keywords :
Experimental model , NAFLD , NASH , Rat , Nonalcoholic Fatty Liver Disease , Diet
Journal title :
Astroparticle Physics
