Author/Authors :
Mohsenzadegan, Monireh Department of Immunology - School of Medicine - Iran University of Medical Sciences, Tehran, Iran , Tajik, Nader Department of Immunology - School of Medicine - Iran University of Medical Sciences, Tehran, Iran , Madjd, Zahra Oncopathology Research Centre and Department of Pathology - School of Medicine - Iran University of Medical Sciences, Tehran, Iran , Shekarabi, Mehdi Department of Immunology - School of Medicine - Iran University of Medical Sciences, Tehran, Iran , Farajollahi, Mohammad M Cellular and Molecular Research Center - Iran University of Medical Sciences, Tehran, Iran
Abstract :
prostate (NGEP), is a prostate-specific gene expressed only in normal prostate and prostate cancer tissue. Because
of its selective expression in prostate cancer cell surface, NGEP is a potential immunotherapeutic target.
To target the NGEP in prostate cancer, it is essential to investigate its expression in prostate cancer cells.
Methods: In the present study, we investigated NGEP expression in LNCaP and DU145 cells by real time and
RT-PCR, flow cytometric and immunocytochemical analyses.
Results: Real time and RT-PCR analyses of NGEP expression showed that NGEP was expressed in the
LNCaP cells but not in DU145 cells. The detection of NGEP protein by flow cytometric and immunocytochemistry
analyses indicated that NGEP protein was weakly expressed only in LNCaP cell membrane.
Conclusion: Our results demonstrate that LNCaP cell line is more suitable than DU145 for NGEP expression
studies; however, its low-level expression is a limiting issue. NGEP expression may be increased by androgen
supplementation of LNCaP cell culture medium.
Keywords :
Prostate cancer , NGEP , LNCaP cell line , DU145 cell line
