Author/Authors :
Hosseini, Mostafa Human Genetics Research Center - Baqiyatallah University of Medical Sciences, Tehran , Mahjoubi, Frouzandeh Department of Clinical Genetic - National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran , Majidzadeh, Tayebeh Department of Clinical Genetic - National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran , Khaje-Hosseini, Fattaneh Human Genetics Research Center - Baqiyatallah University of Medical Sciences, Tehran , Haghipanah, Mahya Human Genetics Research Center - Baqiyatallah University of Medical Sciences, Tehran
Abstract :
BACKGROUND Colorectal cancer (CRC) is one of the most common cancers among men and women worldwide. Cancer metastasis is the main cause of death in patients with cancer. NEBL (nebulette, Gene ID: 10529) protein interacts with thin filaments in the cell and may functionally destabilize focal adhesion composition. There are some studies on NEBL gene expression alteration in cancer. In the presented study we aimed to analyze NEBL gene expression in patients with colorectal cancer to explore possible association of this gene with clinicopathological features in CRC. METHODS
Sixty-seven fresh samples of colorectal tumors and adjacent normal tissues were collected from Iranian patients with CRC. Real time polymerase chain reaction was performed to measure the level of NEBL gene expression and its association with clinico-pathological features. RESULTS A significant overexpression with 3 fold increse was seen in NEBL mRNA level in tumoral tissues compared with the adjacent normal tissues. In addition there was a significant association between NEBL gene expression with lymph node metastasis in patients with CRC. CONCLUSION The overexpression of NEBL has the capacity to be considred as a prognostic biomarker in patients with CRC.
Keywords :
Nebullete , LASP2 , Colorectal cancer , Lymph node metastasis , Prognostic marker
