Author/Authors :
Ghavami, Maryam Department of Anatomical Sciences - Tabriz University of Medical Sciences , Mohammadnejad, Daryoush Drug Applied Research Center - Tabriz University of Medical Science , Beheshti, Rahim Department of Veterinary - Shabestar Branch, Islamic Azad University , Solmani-rad, Jafar Department of Anatomical Sciences , Tabriz University of Medical Sciences , Abedelahi, Ali Department of Anatomical Sciences , Tabriz University of Medical Sciences
Abstract :
Background: Cryopreservation of mammalian ovaries has been reported with different
levels of success. Cryopreservation of ovarian tissue may be a potential alternative
for treatment of infertility and many attempts have been done to improve the
efficiency of ovarian cryopreservation. The objective of the present study was to
compare the direct cover vitrification (DCV) with ethylene glycol (EG), dimethyl
sulfoxide (DMSO) and EG plus DMSO.
Methods: Eighty five mice were sacrificed by cervical dislocation and their ovaries
were cryopreserved in the presence of 5% EG or DMSO alone or as mixture, 10%
EG or DMSO alone or as mixture and a group with ascending concentrations of
cryoprotectants. After toxicity testing and vitrification warming, the ovaries were
fixed for histological and ultrastructural studies. In addition, the viability of mechanically
isolated follicles was studied by trypan blue staining. All data were compared
by ANOVA (p<0.05).
Results: Ovarian tissues frozen in EG plus DMSO in ascending concentrations retained
a higher percentage of morphologically normal and or viable follicles than tissues
frozen in 10 M EG plus DMSO or in either concentration of EG and DMSO
alone (p<0.001). Ultrastructural analysis of ovarian tissues frozen in ascending concentrations
of EG plus DMSO showed that these follicles were well preserved and it
was very similar to the control group.
Conclusion: Cryopreservation of ovarian tissue in EG plus DMSO is the most effective
method for preserving the structural integrity of follicles within the ovary.
