Author/Authors :
Ayatollahi, Hossein Department of Hematology and Blood Bank - Faculty of Medicine - Cancer Molecular Pathology Research Center, Ghaem Medical Center - Mashhad University of Medical Sciences, Mashhad, Iran , Sadeghian, Mohammadhadi Department of Hematology and Blood Bank - Faculty of Medicine - Cancer Molecular Pathology Research Center, Ghaem Medical Center - Mashhad University of Medical Sciences, Mashhad, Iran , Kooshyar, Mohammadmahdi Department of Internal Medicine - Faculty of Medicine - Ghaem Medical Center - Mashhad university of Medical sciences, Mashhad, Iran , Shirdel, Abass Department of Internal Medicine - Faculty of Medicine - Ghaem Medical Center - Mashhad university of Medical sciences, Mashhad, Iran , Rahimi, Hossein Department of Internal Medicine - Faculty of Medicine - Ghaem Medical Center - Mashhad university of Medical sciences, Mashhad, Iran , Jafarian, Amirhossein Department of Pathology - Cancer Molecular Pathology Research Center - Ghaem Medical Center - Mashhad University of Medical Sciences, Mashhad, Iran , Ghazaei, Saeide Cancer Molecular Pathology Research Center - Ghaem Medical Center Mashhad University of Medical Sciences, Mashhad, Iran , Soltani, Narges Cancer Molecular Pathology Research Center - Ghaem Medical Center Mashhad University of Medical Sciences, Mashhad, Iran , Shams, Fatemeh Cancer Molecular Pathology Research Center - Ghaem Medical Center Mashhad University of Medical Sciences, Mashhad, Iran , Motamedi Rad, Neda Cancer Molecular Pathology Research Center - Ghaem Medical Center Mashhad University of Medical Sciences, Mashhad, Iran , Shakeri, Sepideh Cancer Molecular Pathology Research Center - Ghaem Medical Center Mashhad University of Medical Sciences, Mashhad, Iran
Abstract :
Background: Adult T cell leukemia lymphoma (ATLL) is a rare disease, significantly linked to the infection by the human T-cell
lymphotropic virus 1(HTLV-1). ATLL is typically preceded by decades of clinical latency during which infected cells accumulate
selectable traits leading to a malignant transformation. Amongst all the HTLV-1 infected carriers only about 3-5% will develop ATLL.
Despite the intensive attempt to improve the overall survival, ATLL remains one of worse prognosis among the hematologic malignancies.
FMS like tyrosine kinase 3 internal tandem duplication (FLT3-ITD) mutations are mutations which are frequent among leukemic
patients. We aimed to investigate the frequency of FLT3 mutation status in patients with acute type of ATLL which has not
been studied yet.
Methods: In this case control study 38 patients with acute type of ATLL were retrospectively analyzed between February 2015 and
February 2017. Forty HTLV-1 positive patients were also used as control cases. Genomic DNA was extracted according to phenolchloroform
protocol and two restriction fragment length polymorphism (RFLP) PCR reactions were set up to detect FLT3/ ITD and
FLT3/TKD mutations. Differences between variables were evaluated by the chi-square test and t test for categorical and continuous
variables, respectively. SPSS software v. 15 was used for statistical analysis. All P values were two sided and values less than 0.05
were considered to be significant.
Results: No FLT3 mutations were detected in acute type of ATLL patients. So far, not many studies have shown the frequency of
FLT3 mutation in ATLL patients
Conclusion: Therefore, we conclude that although FLT3 mutations are rather unusual in the acute type of ATLL patients, but other
alternative mechanisms associated with ATLL remain to be further investigated. This study was a novel project regarding the analysis
of FLT3 mutation in the field of ATLL research.
