Author/Authors :
Mohammadi, Bahman Student Research Committee - Kurdistan University of Medical Sciences, Sanandaj, Iran , Mohajeri, Parviz Department of Microbiology - School of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Rouhi, Samaneh Student Research Committee - Kurdistan University of Medical Sciences, Sanandaj, Iran , Ramazanzadeh, Rashid Cellular and Molecular Research Center - Research Institute for Health Development - Kurdistan University of Medical Sciences, Sanandaj, Iran
Abstract :
Background: Mutations in embB gene have been reported in ethambutol (EMB) resistance Mycobacterium tuberculosis (M. tuberculosis)
isolates. The aim of this study was survey on embB 306 and 406 EMB resistant M. tuberculosis isolated from patients in West
of Iran (2014-2015).
Methods: Fifty strains of M. tuberculosis from patients with pulmonary tuberculosis (TB) were considered. Drug susceptibility using
proportional method was performed. Polymerase chain reaction (PCR) -DNA sequencing was applied for mutation in embB 306 and
406 codon detection. Data were analyzed in SPSS 16 software using descriptive statistics and Fisher's exact test (p<0.05).
Results: In this study 7 (14%) M. tuberculosis isolates were resistant to EMB. 6 (85.71%) and 1 (14.28%) resistant isolates
had embB 306 and 304 codon mutations, respectively. Between embB306 mutations and resistance to EMB and MDR isolates had a
significant relationship (p<0.001).
Conclusion: The data indicated that embB 306 mutations have effect on EMB resistant. Detection of EMB resistant and these mutations
prominent for antibiotic prescription.
Keywords :
Mycobacterium tuberculosis , Ethambutol Resistance , Mutations , embB gene
