Fabrication of Biosensor for Determination of L-lactate using Elite Nanomaterials based LDH-cMWCNT-MB/Chitosan/SWCNT-Au Electrode

Lactate level being an important parameter is to be monitored in body regularly. During acute conditions it elevation may result in heart failure, hepatic disease, sepsis, tissue hypoxia and many more. A simple and sensitive biosensor has been fabricated by immobilizing lactate dehydrogenase enzyme. It was covalently immobilized on the nanocomposite based working electrode. The developed biosensor showed optimal response at pH 7.5, temperature 30℃, response time was 8s with the substrate concentration of 600 μM, the oxidation peak was obtained at 0.66 V which is at a low potential, Km and Imax values for newly fabricated biosensor were 66.7 μM and 153.8 μA respectively. It possessed lower detection limit of 0.015 mM with storage stability of 3 months. A good correlation has been observed when developed method is compared with standard enzyme kit method proving reliability of the method. Blood samples were successfully screened for lactate concentration using working electrode. The newly developed biosensor is less time consuming, accurate, reliable, less costly, requires no sample pre-treatment, screen large number of samples, suitable for on field determination of lactate concentration and finally reusable due to covalently immobilized enzyme.