Author/Authors :
YOUSEFINEJAD, Abbas Dept. of Nutrition - School of Public Health - Bushehr University of Medical Sciences, Bushehr, Iran , SIASSI, Fereydoon Dept. of Community Nutrition - School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Tehran, Iran , MIRSHAFIEY, Abbas Dept. of Pathobiology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , ESHRAGHIAN, Mohammad-Reza Dept. of Biostatistics - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , KOOHDANI, Fariba Dept. of Cellular and Molecular Nutrition- School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Tehran, Iran , JAVANBAKHT, Mohammad Hassan Dept. of Cellular and Molecular Nutrition- School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Tehran, Iran , SEDAGHAT, Reza Dept. of Anatomy and Pathology - School of Medicine - Shahed University, Tehran, Iran , RAMEZANI, Atena Dept. of Basic Sciences and Nutrition - School of Public Health - Mazandaran University of Medical Sciences, Sari, Iran , ZAREI, Mahnaz Dept. of Cellular and Molecular Nutrition- School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Tehran, Iran , DJALALI, Mahmoud Dept. of Cellular and Molecular Nutrition- School of Nutritional Sciences and Dietetics - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Background: Nephrotic syndrome is a disorder that leads to hyperlipidemia. L-carnitine and genistein can effect on lipid metabolism and the syndrome. In the present study, we have delved into the separate and the twin-effects of L-carnitine and genistein on the gene expressions of HMG-COA reductase and LDL receptor in experimental nephrotic syndrome.
Methods: In this controlled experimental study, 50 male Sprague–Dawley rats were randomly divided into five groups: NC (normal-control), PC (patient-control), LC (L-carnitine), G (genistein), LCG (L-carnitine-genistein). Adri-amycin was used for inducing nephrotic syndrome and the spot urine samples and urine protein-to-creatinine ratio were measured. Hepatocytic RNA was extracted and real-time PCR was used for HMG-COA Reductase and LDL receptor gene Expression measurement.
Results: The final weight of the patients groups were lower than the NC group (P=0.001), and weight gain of the NC group was higher than the other groups (P<0.001). The proteinuria and urine protein-to-creatinine ratio showed sig-nificant differences between PC group and LC, G and LCG groups at week 7 (P<0.001). The expression of HMG-COA Reductase mRNA down regulated in LC, G and LCG groups in comparison with PC group (P<0.001). ΔCT of LDLr mRNA showed significant differences between the PC group and the other patient groups (P<0.001).
Conclusion: This study shows a significant decreasing (P<0.001) and non-significant increasing trend in HMG-COA Reductase and LDLr gene expression, respectively, and synergistic effect of L-carnitine and genistein on these genes in experimental nephrotic syndrome.
Keywords :
Gene , Genistein , L-carnitine , LDL Receptor , Nephrotic syndrome
