Title of article :
Sequence Variation in Measles Virus F Gene of AIK-C Vaccines Strain after Passage in Vero Cells
Author/Authors :
Azita- Sadigh, Zohreh Virology Department of Iran University of Medical Sciences - Razi Vaccine and Serum Research Institute , Shamsi Shahrabadi, Mahmoud Virology Department of Iran University of Medical Sciences , Shafyi, Abbas Razi Vaccine and Serum Research Institute , Bambai, Bijan National Institute of Genetic Engineering and Biotechnology
Abstract :
The live attenuated AIK-C measles virus which is used for vaccine to control measles in the world has considerably reduced the incidence of this disease. In the present study we have cloned and analyzed the sequence of the cDNA coding for the F protein of AIK-C Vaccine Strain of Measles Virus after limited passage in Vero Cells. Comparison studies demonstrated a high degree of nucleotide sequence homology (98%) to the AIK-C vaccine strain.Altogether four mutations were observed compared to the sequence of AIK-C vaccine strain which was reported by Mori et al and deposited in GeneBank. These mutations caused amino acid changes at position 278, 362, 419 and 549. Change from leucine to phenylalanine at position 278 of the protein that was an early event during adaptation to Vero cells, and also changes at position 362 and 419 were reported by previous study but change at position 549 wasn't report before and must be study more.
Keywords :
Measles Virus , AIK-C vaccine strain , F gene , pDONR221 , Restriction Endonuclease
Journal title :
Astroparticle Physics
