Author/Authors :
Ghorbani, S. G. M Iranian Research Institute of Plant Protection - Dept. of Plant Virology, Tehran - University of Guilan - Dept. of Plant Protection, Rasht , Shahraeen, N Iranian Research Institute of Plant Protection - Dept. of Plant Virology, Tehran , Elahinia, S. A University of Guilan - Dept. of Plant Protection. Rasht.
Abstract :
Damage to cowpea crops by different viruses has occurred in Iran in the past. A total of 119 symptomatic samples were collected from cowpea fields throughout Guilan province, Iran during the summer 2006 and 10 viruses tested by Double Antibody Sandwich Enzyme Linked Immuno Sorbent Assay (DAS-ELISA), 7 viruses tested by Tissue Blot Immuno Assay Antigen Coated-Plate (ACP-ELISA) used for detection of the Potyviruses. Using DAS-ELISA Bean leaf Roll Virus-BLRV (10%), Cowpea Mild Mottle Virus-CpMMV (4%), Cowpea Mottle Virus-CpMoV (6%), Cowpea Mosaic Virus-CpMV (10%), Southern Bean Mosaic Virus-SBMV (1%), Cucumber Mosaic Virus-CMV (13%), Cowpea Aphid Borne Mosaic Virus-CABMV (0.8%), Bean Yellow Mosaic Virus-BYMV (3%), Cowpea Chlorotic Mottle Virus-CCMV (2%) was test, the samples were shown infection to Cowpea Severe Mosaic Virus-CpSMV (13%), Bean Common Mosaic Virus-BCMV (25%), Alfalfa Mosaic Virus-AMV (12%), Bean Pod Mottle Virus-BPMV (5%), Clover Yellow Vein Virus-CYW (2%), Peanut Stunt Virus-PSV (6%) and Blackeye Cowpea Mosaic Virus-BICMV (12%) and 12% to group specific potyviruses Mab. were detected with ACP - ELISA. No positive reactions were found with Bean Golden Mosaic Virus-BGMV. BCMV was found to be more prevails in Guilan province. Mixed infection by viruses was observed in many samples.
Keywords :
Cowpea , Virus diseases , Serology , Incidence
