Author/Authors :
Kazemi, F Parasitology Dept. - School of Medicine - Kashan University of Medical Sciences, Kashan, Iran , Hooshyar, H Parasitology Dept. - School of Medicine - Kashan University of Medical Sciences, Kashan, Iran , Zareikar, B Parasitology Dept. - School of Medicine - Tabriz University of Medical Sciences, Tabriz, Iran , Bandehpour, M Cellular and Molecular Biology Research Center - Shahid Beheshti University, M.C., Tehran, Iran , Arbabi, M Parasitology Dept. - School of Medicine - Kashan University of Medical Sciences, Kashan, Iran , Talari, S Parasitology Dept. - School of Medicine - Shahid Beheshti University, M.C., Tehran, Iran , Alizadeh, R Parasitology Dept. - School of Medicine - Kashan University of Medical Sciences, Kashan, Iran , Kazemi, B Cellular and Molecular Biology Research Center - Shahid Beheshti University, M.C., Tehran, Iran
Abstract :
Background: Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a
choice drug for its treatment. Because of disease importance in public health and its controversial
ideas about the prevalence of drug resistance, this study was carried out.
Methods: Fifty-two suspected vaginal samples were collected from 2006 to 2007 in Gynecology
Maryam Hospital, Tehran, Iran. All isolates were examined by microscopic, culture and PCR
techniques. The PCR products were analyzed by RFLP and CSGE methods and two suspected
samples were sequenced.’
Results: Trichomonas vaginalis was identified from all 52 samples. Of 52 isolates, 45 samples
were successfully cultured and amplified by PCR except one. Seven were positive only by PCR.
Finally, ITS1 fragment was successfully amplified in 51 of 52. CSGE analysis and PCR products
digestion by MspI followed by sequencing showed nucleotide mutation at position 209 (C209T)
of the ITS1 fragment in two (3.9%) of themú
Conclusion: The results showed mutation in ITS1 fragment of T. vaginalis in two (3.9%) of Iranian
isolates which may be related to metronidazole resistance.
