Author/Authors :
EBRAHIMIPOUR, Mohammad Dept. of Parasitology and Mycology - School of Medicine - Shiraz University of Medical Sciences, Shiraz, Iran , SADJJADI, Mahmoud Dept. of Parasitology and Mycology - School of Medicine - Shiraz University of Medical Sciences, Shiraz, Iran , YOUSOFI DARANI, Hossein Dept. of Parasitology and Mycology - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , NAJJARI, Mohsen Dept. of Parasitology and Mycology - School of Medicine - Shiraz University of Medical Sciences, Shiraz, Iran
Abstract :
Background: Cystic echinococcosis (CE) is one of the most important zoonotic
diseases; caused by different genotypes of Echinococcus spp. Camels have an important
role in transmission cycle of E. granulosus especially, in desert areas. This study
aimed to investigate molecular characterization of hydatid cysts isolates from onehumped
camel (Camelus dromedarius) and to show its molecular and phylogenic status
in this important CE host in the central part of Iran.
Methods: Twenty hydatid cyst samples (14 fertile and 6 calcified) were collected
from 56 slaughtered camels in Central part of Iran. Extraction of DNA from 14
fertile samples was achieved followed by molecular studies on two mitochondrial
genes (nad1 and cox1).
Results: Blast and phylogenetic analysis on sequenced genes showed the presence
of G1 (28.6%), G3 (28.6%) and G6 (35.7%) genotypes in the samples. However,
one sample was detected as E. ortleppi (G5) with 99% homology with G5 isolated
from camel in Egypt (AB921055) and Sudan (JX912709).
Conclusion: Presence of E. ortleppi, originally the cattle genotype, is reported for
the first time in Iran. Due to the potential of infecting human by E. ortleppi; more
attention should be paid to this zoonotic genotype in this region.
Keywords :
Echinococcus ortleppi , Mitochondrial DNA genes , Phylogenetic analysis , Camel , Iran
