Author/Authors :
JEIBOUEI, Shabnam Dept. of Parasitology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , BANDEHPOUR, Mojgan Cellular and Molecular Biology Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , KAZEMI, Bahram Cellular and Molecular Biology Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , HAGHIGHI, Ali Dept. of Parasitology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Background: Leishmaniasis is a neglected disease affecting millions of people
worldwide. The treatment of the disease is hampered due to high cost, toxicity and
the crisis of drug resistance. Polytope approaches of genetic immunization could
be a strategy for prevention of infectious diseases. Furthermore, the identification
of Leishmania genome sequence and the application of bioinformatics assist us to
devise an effective vaccine’s candidate.
Methods: A linear sequence from predicted epitopes of GP63, LACK and CPC
antigens was designed and was optimized using online available algorithms. The
synthesized sequence (LAKJB93) was ligated to pEGFP-N1 plasmid.
Results: The 264bp sequence was cloned at N terminal of GFP into pEGFP_N1
expression vector and transfect into CHO cell line. Construct was efficient expressed
in CHO cells.
Conclusion: The protein of LAKJB93 cosnstruct was expressed in CHO cells successfully.
