Author/Authors :
SHOKRI, Azar Molecular and Cell Biology Research Center - Dept. of Parasitology - School of Medicine - Mazandaran University of Medical Sciences, Sari, Iran , SAEEDI, Majid Dept. of Pharmaceutics - School of Pharmacy - Mazandaran University of Medical Sciences, Sari, Iran , FAKHAR, Mahdi Dept. of Medicinal Chemistry - School of Pharmacy - Mazandaran University of Medical, Sciences, Sari, Iran , MORTEZA-SEMNANI, Katayoun Dept. of Medicinal Chemistry - School of Pharmacy - Mazandaran University of Medical, Sciences, Sari, Iran , KEIGHOBADI, Masoud Students Research Committee - School of Pharmacy - Mazandaran University of Medical Sciences, Sari, Iran , HOSSEINI TESHNIZI, Saeed nfectious and Tropical Diseases Research Center - Hormozgan University of Medical Sciences, Bandar Abbas, Iran , KELIDARI, Hamid Reza Dept. of Pharmaceutics - School of Pharmacy - Mazandaran University of Medical Sciences, Sari, Iran , SADJADI, Siamak Students Research Committee - School of Medicine - Mazandaran University of Medical Sciences, Sari, Iran
Abstract :
Background: The aim of present study was to evaluate antileishmanial effects of Lavandula
angustifolia (L. angustifolia) and Rosmarinus officinalis (R. officinalis) medicinal plants essential oils
and nano-emulsions on Leishmania major (L. major).
Methods: The present study was performed in Leishmaniasis Reference Lab at Mazandaran
University of Medical Sciences, Iran during 2016-2017. The IC50 values were calculated
in both the promastigote and amastigote stages in J774 macrophage in comparison with
meglumine antimoniate (MA) as positive control. In addition, cytotoxicity effects of essential
oils and nano-emulsions prepared from both plants against macrophages were evaluated.
Results: Both essential oil and nano-emulsion of Lavander and Rosemary showed antileishmania
activity on promastigote with IC50=0.11 μl/mL, IC50=0.26 μl/mL, and
IC50=0.08 μl/mL respectively. Moreover, during amastigote assay, Lavander and Rosemary
essential oils and nano-emulsion were effective at least in concentration of 0.12 μl/mL and
0.06 μl/mL (P=0.0001) respectively, on mean infected macrophages (MIR) and amastigotes
in macrophages (P=0.0001). Additionally, cytotoxicity assay against macrophage revealed no
toxicity on the host cells at IC50 concentrations.
Conclusion: The nano-emulsions of both plants were more effective than essential oil in
both MIR and amastigote. However, in comparison with MA, the Lavander essential oil is
more effective in reducing MIR. Rosemary nano-emulsion reduced MIR significantly more
than MA in concentration of 0.25 μl/mL (P<0.001). Further investigations are recommended
to evaluate the effect of these medicinal plants in murine models.
Keywords :
Leishmania major , Lavandula angustifolia , Rosmarinus officinalis , Essential oil , Nano-emulsion
