Title of article :
Phylogenetic Analysis of C Type Lectin from Toxocara canis Infective Larvae and Comparison with the C Type Lectin Family in the Immune System of Mouse and Human
Author/Authors :
ETEBAR, Fazeleh Dept. of Parasitology - Faculty of Veterinary Medicine - University of Tehran, Tehran, Iran , HOSSEINI, Hossein Dept. of Parasitology - Faculty of Veterinary Medicine - University of Tehran, Tehran, Iran , JALOUSIAN, Fatemeh Dept. of Parasitology - Faculty of Veterinary Medicine - University of Tehran, Tehran, Iran , RANJBAR, Mohammad Mehdi Dept. of Immunology - Razi Vaccine and Serum Research Institute, Karaj, Iran
Abstract :
Background: C type lectin (CTL) family is a type of calcium-dependent proteins
found in vertebrates and invertebrates. The objective of this study was to perform a
comparative analysis and phylogenetic inferring for understanding the similarities and
differences of carbohydrate recognition domain (CRD) domain of Toxocara canis CTL
and other nematodes, and similar C type lectin involved in the immune system of
mouse and human as their host.
Methods: The female T. canis was retrieved from the 2-6 months puppies (Department
of Parasitology, Faculty of Veterinary Medicine, University of Tehran, 2015). To collect
T. canis eggs, the worms were cultured for 5 d until they were embryonated. The hatching
process was accelerated for collecting the stage 2 larvae, and the larvae were cultured
for a week. A cDNA library was made from the total mRNA of T. canis infective
larvae. The PCR amplification for C type lectin gene was performed and the amino
acids were analyzed using the alignment method and the construction of phylogenetic
tree.
Results: The suspension sample maintained at 30 ºC for four weeks could embryonate
90%-100% of eggs. T. canis CTL gene was 657 bp in length and encoded a protein with
219 amino acids. The CTL of species of Strongylida order were closely placed in the
tree, whereas the members of Ascaridida orders were located in a separate branch.
High levels of similarity (36%-44%) and conservation of C type lectin from T. canis
with mouse and human C type lectins. Its C type lectin showed a higher similarity with
asialoglycoprotein receptor (ASGPR), macrophage lectin, dendritic cell-specific intercellular
adhesion molecule 3-grabbing nonintegrin (DC-SIGN), MINCLE receptor of
mouse and human.
Conclusion: Analysis of CRD domain of C type lectin protein could make a better
understanding of their role in the interaction of nematode parasite with their hosts.
Keywords :
Nematoda , Toxocara canis , C type lectin , Phylogenetic analysis
Journal title :
Astroparticle Physics
