Author/Authors :
SARKARI, Bahador Basic Sciences in Infectious Diseases Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , MANSOURI, Majid Basic Sciences in Infectious Diseases Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , NOORPISHEH GHADIMI, Shamsi Basic Sciences in Infectious Diseases Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , ABDOLAHI KHABISI, Samaneh Dept. of Parasitology and Mycology - Faculty of Medicine - Zahedan University of Medical Sciences, Zahedan, Iran , DOSHMANZIARI, Abdolla Basic Sciences in Infectious Diseases Research Center - Shiraz University of Medical Sciences, Shiraz, Iran
Abstract :
Wild boars may be infected with several zoonotic parasitic infections including
Fasciola spp. We reported a case of Fasciola infection in a wild boar in Bushehr
Province in southwestern Iran. The sample was isolated from the liver of a hunted
wild boar. A few of adult worms were fixed and stained. DNA was extracted from
apical and lateral parts of the worms and PCR amplified, targeting NADH dehydrogenase
subunit 1 (nad1) and cytochrome C oxidase subunit 1 (cox1) mitochondrion
genes. Although the worm was quite long and looked much similar to F.
gigantica, sequencing and analysis of PCR products of nad1 and cox1 genes revealed
that the isolate has the most similarity with F. hepatica. This is the first report of
molecular evaluation of Fasciola spp. from wild boar in Iran.
