Author/Authors :
ELIKAEE, Samira Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , MOHEBALI, Mehdi Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , ESLAMI, Hamid Dept. of Medical Biotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran, Iran , REZAEI, Sassan Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , NAJAFIAN, Hamid Reza Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , KAZEMI-RAD, Elham Dept. of Parasitology - Pasteur Institute of Iran, Tehran, Iran , KESHAVARZ, Hossein Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , ESHRAGHIAN, Mohammad Reza Dept. of Biostatistics and Epidemiology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , HAJJARAN, Homa Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , OSHAGHI, Mohammad Ali Dept. of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , AYAZIAN MAVI, Sara Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Background: Cutaneous leishmaniasis (CL) is one of the world health problems.
Leishmania major is the etiological agent of zoonotic cutaneous leishmaniasis (ZCL).
Promastigote and amastigote are two morphological forms of Leishmania parasites
that express different proteins and p27 is an important gene encoding cytochrome
c oxidase (COX) component. P27 gene expresses a 27 kDa protein that essential in
ATP synthesis. This study aimed to compare p27 gene expression in promastigote
and amastigote forms in Iranian strain of L. major (MRHO/IR/75/ER).
Methods: This study was conducted in 2015. Clinical isolates of CL patients from
north, center, west and south parts of Iran were collected and identified by PCRRFLP.
After RNA extraction of promastigotes and amastigotes and cDNA synthesis,
the expression level of p27 gene was compared by real-time RT-PCR.
Results: By comparison of expression level between amastigote and promastigote
forms of Iranian strain of L. major, up-regulation of p27 gene (2.73 fold) was observed
in amastigotes. Moreover, there was no significant difference in p27 gene
expression between L. major isolates.
Conclusion: p27 gene and protein can be considered as a target in recombinant
vaccine production and treatment process.
Keywords :
p27 gene , Real-time RT-PCR , Leishmania major , Amastigote , Promastigote
