Author/Authors :
TAVAKOLI KARESHK, Amir Infectious Diseases Research Center - Birjand University of Medical Sciences, Birjand, Iran , TAVAKOLI OLIAEE, Razieh Dept. of Medical Parasitology and Mycology - School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , MAHMOUDVAND, Hossein Razi Herbal Medicines Research Center - Lorestan University of Medical Sciences, Khorammabad, Iran , KEYHANI, Amir Dept. of Medical Parasitology and Mycology - School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , MOHAMMADI, Mohammad Ali Dept. of Medical Parasitology and Mycology - School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , BAMOROVAT, Mehdi Dept. of Medical Parasitology and Mycology - School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , HAJHOSSEINIMohammad Ali School of Veterinary Medicine - Kerman University of Medical Sciences, Kerman, Iran , ZIA-ALI, Naser Dept. of Medical Parasitology and Mycology - School of Medicine - Kerman University of Medical Sciences, Kerman, Iran
Abstract :
Background: We isolated Toxoplasma gondii from camels by bioassay method in mice
model and detect parasitic DNA in brain mice by molecular methods.
Methods: One hundred tissue samples including heart (n=50), and diaphragm (n=50)
were collected from camels (n=50) slaughtered in abattoirs from Feb to Oct 2015 in
three provinces located in eastern Iran. In first, blood sample from 50 camels was assayed
for anti-Toxoplasma antibodies by modified agglutination test (MAT) test. Bioassay
method was done in positive MAT blood camels in BALB/c mice and Nested PCR
performed in seropositive tissue samples to amplify the B1 and GRA6 genes. The existence
of polymorphic restriction sites for endonuclease MseI was used with PCRRFLP
method and Sequencing analysis to evaluate the prevalence of type strains (I, II
and III)
Results: Overall, 13 (26%) of camels were positive with titer of 1:20 for toxoplasmosis
and 13(26%) tissue samples of camels were found positive for the T. gondii B1 gene,
including 7(14%) diaphragm, 6(12%) heart. Moreover, 3(6%) tissue samples of camels
were found positive with GRA6 gene for T. gondii. There are three genotypes and mix
genotype using MseI enzyme among all positive samples.
Conclusion: The obtained results from serological and molecular tests demonstrated
the infection of T. gondii with previously recognized genotypes in the tissues of camels
for first time from Iran. Since consumption of meat camels are raising in Iran, there
may be a high risk of toxoplasmosis through consumption of products from these
hosts due to their susceptibility to the infection.
Keywords :
Toxoplasma gondii , Isolation , Bioassay , Genotyping , PCR-RFLP
