Author/Authors :
ABOLGHAZI, Ahmad Dept. of Parasitology and Mycology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , HEIDARI, Aliehsan Dept. of Medical Parasitology - School of Medicine - Alborz University of Medical Sciences, Karaj, Iran , MOIN-VAZIRI, Vahideh Dept. of Parasitology and Mycology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , HAGHIGHI, Ali Dept. of Parasitology and Mycology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , SEYYED TABAEI, Javad Dept. of Parasitology and Mycology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , KESHAVARZ, Hossein Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , SHOJAEE, Saeedeh Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Background: Vivax malaria is more prevalent in the malarious areas of Iran,
which makes vaccine research a high priority. Serine Repeat Antigens (SERA)
have essential role in the parasite life cycle and high expression profiles of
PvSERA5 make it suitable vaccine candidates. This study aimed to evaluate the
genetic diversity of C-terminal region of PvSERA5 in Iranian isolates of Plasmodium
vivax in Sistan and Baluchistan.
Methods: Totally, 49 blood samples were taken from symptomatic malaria patients
in Sistan and Baluchistan Province in 2016. Mono-infection to P. vivax
was confirmed by 18srRNA-Nested-PCR. Genomic DNA was extracted and Cterminal
region of PvSERA5 was amplified by specific primers. PCR-products
have been sequenced and analysis was done by using bioinformatics software,
mainly DnaSP & MEGA5.
Results: Genetic diversity was calculated 14.8% in C-terminal region of
PvSERA5 in Iranian isolates, 19 different sequences and 4 haplotypes existed.
The amount of Tajima’s D (0.3805) and ratio of non-synonymous to synonymous
mutation (1.82) showed that C-terminal region of PvSERA5 is under positive
natural selection; also intragenic recombination could interfere.
Conclusion: Results could be helpful in any research, regarding this antigen as
vaccine candidate in Iran or worldwide.
Keywords :
Plasmodium vivax , PvSERA5 , Genetic diversity , Iran
