Author/Authors :
Hejazi, SH Dept. of Parasitology and Mycology - School of Medicine - Esfahan University of Medical Sciences , Zia Jahromi, N Science and Research Campus - Islamic Azad University Tehran , Bandehpour, M Cellular and Molecular Biology Research Center - Shahid Beheshti University , Eslami, G Dept. of Parasitology and Mycology - School of Medicine - Esfahan University of Medical Sciences , Salehi, R Dept. of Genetic and Molecular Biology - School of Medicine - Esfahan University of Medical Sciences , Khamesipour, A Center of Research in Skin diseases and Leprosy - Tehran University of Medical Science , Kazemi, B Dept. of Parasitology and Mycology - School of Medicine - Esfahan University of Medical Sciences
Abstract :
Background: Leishmania is an obligatory intracellular protozoan parasite, which infects human beings when infected sand fly vector takes a blood meal. Most efforts are towards designing an effective vaccine to prevent leishmaniasis. In this way, development of candidate antigen for vaccine has special important. In this study, we cloned mannose-1-phosphate guanyltransferase gene of Iranian L .major in pET32a expression vector. Methods: Primers based on L. major mannose-1-phosphate guanyltransferase sequence gene was designed and synthesized. DNA of Leishmania promastigotes was extracted and PCR reaction was done. PCR product was cloned into pTZ57R and sub cloned into pET32a expression vector. Results: Recombinant plasmid containing 1140 bp as L. major mannose-1-phosphate guanyltransferase gene was extracted and confirmed by restriction analysis. PCR product was sequenced and deposited to GenBank. There were some differences in amino acid sequences between Iranian L. major mannose-1-phosphate guanyltransferase and others previously accepted in GenBank Conclusion: We amplified and cloned Iranian L. major mannose-1-phosphate guanyltransferase successfully.
