Author/Authors :
Ashraf, Mohammad Javad Transplant Research Center - Department of Pathology - Shiraz Medical School - Shiraz University of Medical Sciences, Shiraz, IR Iran , Makarempour, Alireza Transplant Research Center - Department of Pathology - Shiraz Medical School - Shiraz University of Medical Sciences, Shiraz, IR Iran , Monabati, Ahmad Transplant Research Center - Department of Pathology - Shiraz Medical School - Shiraz University of Medical Sciences, Shiraz, IR Iran , Azarpira, Negar Transplant Research Center - Department of Pathology - Shiraz Medical School - Shiraz University of Medical Sciences, Shiraz, IR Iran , Khademi, Bijan Department of Otolaryngology - Shiraz Medical School - Shiraz University of Medical Sciences, Shiraz, IR Iran , Hakimzadeh, Afsoon Transplant Research Center - Department of Pathology - Shiraz Medical School - Shiraz University of Medical Sciences, Shiraz, IR Iran , Abedi, Elham Transplant Research Center - Department of Pathology - Shiraz Medical School - Shiraz University of Medical Sciences, Shiraz, IR Iran , Valibeigi, Bita Transplant Research Center - Department of Pathology - Shiraz Medical School - Shiraz University of Medical Sciences, Shiraz, IR Iran
Abstract :
Background: Epstein Barr Virus (EBV) is one of the most common viral infections in human population. EBV has a significant role in
pathogenesis of Hodgkin's lymphoma, Burkitt's lymphoma and nasopharyngeal carcinoma. The role of EBV in non-Hodgkin’s lymphoma,
diffuse large B cell (NHL - DLBL) in the head and neck is controversial. Objectives: The purpose of this study is to find out the difference between the presence of Epstein Barr virus in nodal and extra nodal
lymphoma of head and neck. Patients and Methods: A total of 30 cases of DLBL in two separate groups were collected from pathology department. The first group was
consisted of 15 patients with DLBL of neck lymph node and the other was consisted of 15 patients with extra nodal DLBL of head and neck
mainly in palatine tonsil. Both immune-histo-chemical (IHC) study and polymerase chain reaction (PCR) for detection of late membrane
antigen (LMP) were performed on formalin fixed paraffin embedded tissue. Results: All 30 cases were negative for EBV in IHC method. But in PCR method, 10% of patients were positive for LMP gene. There were 2 positive
cases in nodal lymphoma and 1 positive case in extra nodal lymphoma group. Conclusions: Compare with PCR method, it seems that IHC is not a sensitive method for detection of EBV. Overall, the finding of EBV in NHL
depends on site, type of lymphoma and the detection method.
Keywords :
Epstein-Barr Virus Infections , Lymphoma , Non-Hodgkin , Head and Neck , Antigens
