Author/Authors :
Sadeghi, Iman Department of Genetics - Faculty of Biological Sciences - Tarbiat Modares University, Tehran, IR Iran , Yousefzadi, Morteza Department of Biology - Faculty of Basic Sciences - Hormozgan University, Bandar Abbas, IR Iran , Behmanesh, Mehrdad Department of Genetics - Faculty of Biological Sciences - Tarbiat Modares University, Tehran, IR Iran , Sharifi, Mozafar Department of Plant Biology - Faculty of Biological Sciences - Tarbiat Modares University, Tehran, IR Iran , Moradi, Aiuob Agricultural and Natural Resources Research Cener, Gilan, IR Iran
Abstract :
Background: Many members of the genus Satureja have aromatic and medicinal characteristics. Objectives: The purpose of the present work was to determine cytotoxic activity of the essential oil of S. intermedia CA Mey (Lamiaceae) on two
human cancerous cell lines and its in vitro inhibitory effects against 11 pathogenic bacteria and fungi as well. Materials and Methods: The essential oil was isolated by hydrodistillation and analyzed by combination of capillary GC-FID and GC-MS. The
in vitro toxicological study was based on the MTT cytotoxicity assay and antimicrobial activity of the essential oil was studied according to the
disc diffusion method and MIC value. Results: Thymol (34.5%), γ-terpinene (18.2%) and ρ-cymene (10.5%) were the main components of the essential oil. The toxicological study on
5637 and KYSE cell lines showed IC50 values of 156 μg/ml. The essential oil exhibited considerable antimicrobial activity on tested bacteria and
fungi. Conclusions: From the results of the present study, it may be concluded that the essential oil of S. intermedia and its major constitutes are
interesting in antibacterial and anticancer applications.
