Author/Authors :
Bakhtiary, Mehrdad Cellular & Molecular Research Center - Tehran University of Medical Sciences - Department of Anatomy - Tehran University of Medical Science , Marzban, Mohsen Cellular & Molecular Research Center - Tehran University of Medical Sciences - Department of Anatomy - Tehran University of Medical Science , Mehdizadeh, Mehdi Cellular & Molecular Research Center - Tehran University of Medical Sciences - Department of Anatomy - Tehran University of Medical Science , Joghataei, Mohammad Taghi Cellular & Molecular Research Center - Tehran University of Medical Sciences - Department of Anatomy - Tehran University of Medical Science , Khoei, Samideh Cellular & Molecular Research Center -Tehran University of Medical Sciences - Department of Medical Physics - Tehran University of Medical Sciences , Tondar, Mahdi Department of Anatomy - Tehran University of Medical Science , Pirhajati Mahabadi, Vahid Department of Anatomy - Tehran University of Medical Science , Laribi, Bahareh Department of Immunology - Tehran University of Medical Sciences , Ebrahimi, Asghar Department of Biotechnology and Bioscience - Milano-Bicocca University - Milan, Italy , Hashemian, Jafar Department of Anatomy - Tehran University of Medical Science , Modiry, Navid Department of Anatomy - Tehran University of Medical Science , Mehrabi, Soraya Department of physiology - Tehran University of Medical Sciences
Abstract :
Objective(s) Clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to examine the impression of a combination therapy. This was performed by intravenous injection of human umbilical cord matrix stem cell (hUCMSC-Wharton,s jelly stem cell) with bone marrow cell mobilized by granulocytecolony stimulating factor (G-CSF) in rats injured with cortical compact device. Materials and Methods Adult male Wistar rats (n= 50) were injured with controlled cortical impact device and divided into five groups. All injections were performed 1 day after injury into the tail veins of rats. Neurological functional
evaluation of animals was performed before and after injury using modified neurological severity scores (mNSS). Animals were sacrificed 42 days after TBI and brain sections were stained by Brdu immunohistochemistry. Results Statistically significant improvement in functional outcome was observed in treatment groups when compared with control (P< 0.01). mNSS showed no significant differences among the hUCMSC and G-CSF treated groups at any time point (end of trial). Rats with hUCMSC + G-CSF treatment had a significant improvement on mNSS at 5 and 6 week compared to other treatment group (P< 0.01). Conclusion Histological analysis in G-CSF+ hUCMSC treated traumatic rats exhibited significant increase in numbers of
Brdu immunoreactive cells in their traumatic core compared with other labeled group.
Keywords :
Combine Therapy , G-CSF , Stem Cell , TBI , Wharton,s jelly
