Author/Authors :
Dezfulian, Anahita Research Centre of Gastroenterology and Liver Diseases (RCGLD) in Shahid Beheshti University, Tehran , Aslani, Mohammad Mehdi Department of Microbiology - Institute Pasteur of Iran, Tehran , Oskoui, Mahvash Department of Microbiology - Institute Pasteur of Iran, Tehran , Farrokh, Parisa Department of Microbiology - Institute Pasteur of Iran, Tehran , Azimirad, Masumeh Research Centre of Gastroenterology and Liver Diseases (RCGLD) in Shahid Beheshti University, Tehran , Dabiri, Hossein Research Centre of Gastroenterology and Liver Diseases (RCGLD) in Shahid Beheshti University, Tehran , Salehian, Mohammad Taghi Research Centre of Gastroenterology and Liver Diseases (RCGLD) in Shahid Beheshti University, Tehran , Zali, Mohammad Reza Research Centre of Gastroenterology and Liver Diseases (RCGLD) in Shahid Beheshti University, Tehran
Abstract :
Objective(s) Staphylococcus aureus is a common cause of human infection, and emergence of vancomycin-resistance S. aureus is a great concern for treatment of methicillin-resistant S. aureus,(MRSA) in recent years (MRSA). Here, we report the isolation of high-level VRSA. Materials and Methods S. aureus was isolated from foot ulcer of a diabetic woman in Tehran, Iran. Antibiotic susceptibility was determined according to CLSI guidelines. VanA gene cluster PCR was carried out and PCR amplicon of vanA was sequenced. Results S. aureus had high-level vancomycin-resistant (MIC 512 ≥ μg/ml). Patient's history revealed that VRSA isolate was acquired through community transmission. Only vanA, vanR and vanS genes were amplified in
our isolate. Sequencing revealed that the vanA sequence had high similarity to the vanA sequence of Tn1546. Conclusion Although VRSA infection continues to be rare, isolation of community–acquired VRSA is a significant issue and it needs the efforts of public health authorities.
