Title of article :
Study the Antinociceptive Effect of Intracerebroventricular Injection of Aqueous Extract of Origanum Vulgare Leaves in Rat: Possible Involvement of Opioid System
Author/Authors :
Pahlavan, Yasaman Neuroscience Research Center and Department of Physiology - Faculty of Medicine - Kerman University of Medical Sciences , Sepehri, Gholamreza Neuroscience Research Center and Department of Physiology - Faculty of Medicine - Kerman University of Medical Sciences , Sheibani, Vahid Neuroscience Research Center and Department of Physiology - Faculty of Medicine - Kerman University of Medical Sciences , Afarinesh khaki, Mohammadreza Department of Physiology - Faculty of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Gojazadeh, Morteza Departments of Physiology - Faculty of Medicine - Tabriz University of Medical Sciences , Pahlavan, Bahare Faculty of Medicine - Tabriz University of Medical Sciences , Pahlavan, Fereshteh Faculty of Medicine - Ardabil Azad University of Medical Sciences
Abstract :
Objective(s): The aim of study was to investigate the antinociceptive effect of intracerebroventricular (ICV) microinjection of Origanum vulgare (ORG) extract and possible involvement of opioid receptors. Materials and Methods: Cannula was inserted into left ventricle of male rats. Five days after surgery Tail Flick Latency (TFL) was measured after ICV microinjection of, ORG (1, 3 and 6 μg / rat). Effective dose of ORG was injected ICV in concomitant with morphine (2 mg/kg, IP), naloxone (2 mg / kg, IP) and saline (0.5 μl/rat) and TFL was recorded. Results: The co- administration of ORG extract with morphine showed a significant increase in TFL and naloxone, pretreatment significantly inhibited the antinociceptive activity of ORG and morphine.
Conclusion: The aqueous extract of ORG possesses antinociceptive activities in the tail-flick test in a dose dependent manner. ORG - induced antinociception may have been mediated by opioid systems.
Keywords :
Intracerebroventricular , Origanum vulgare , Pain , Rat , Tail-flick test
Journal title :
Astroparticle Physics
