Author/Authors :
Vahabi, Farideh Biochemistry Department - Pasteur Institute of Iran - Pasteur Avenue, Tehran , Sadeghi, Sedigheh Amir Alam Hospital - Amir Alam, Tehran , Arjmand, Mohammad Biochemistry Department - Pasteur Institute of Iran - Pasteur Avenue, Tehran , Mirkhani, Fatemeh Biochemistry Department - Pasteur Institute of Iran - Pasteur Avenue, Tehran , Hosseini, Eshagh Amir Alam Hospital - Amir Alam, Tehran , Mehrabanfar, Mahsheed Payam Noor University, Tehran , Hajhosseini, Reza Payam Noor University, Tehran , Iravani, Ayda Biochemistry Department - Pasteur Institute of Iran - Pasteur Avenue, Tehran , Bayat, Parastou Biochemistry Department - Pasteur Institute of Iran - Pasteur Avenue, Tehran , Zamani, Zahra Biochemistry Department - Pasteur Institute of Iran - Pasteur Avenue, Tehran
Abstract :
Objective(s): Determination of stages of colon cancer is done by biopsy usually after surgery. Metabolomics is the study of all the metabolites using LC-MS and 1HNMR spectroscopy with chemometric techniques. The stages of colon cancer were detected from patients' sera using 1HNMR. Materials and Methods: Five ml blood was collected from 16 confirmed patients referred for colonoscopy. One group of eight patients were diagnosed with stage 0 to I colon cancer and the second group of 8 patients with II-IV stage colon cancer. Sera were sent for 1HNMR. The differentiating metabolites were identified using HMDB and the metabolic cycles from Metaboanalyst. Results: Six metabolites of which pyridoxine levels lowered, and glycine, cholesterol, taurocholic acid, cholesteryl ester and deoxyinosine increased. Conclusion: The different stages of cancer were identified by the main metabolic cycles such as primary bile acid biosynthesis, purine and vitamin B metabolic pathways and the glutathione cycle.
Keywords :
Colon cancer , 1HNuclear Magnetic Resonance Spectroscopy , Metabolomics , Serum , Staging
