Author/Authors :
Farhadian, S Department of Biology, University of Shahrekord , Shareghi, B Department of Biology, University of Shahrekord , Salavati-Niasari, M Institute of Nano Science and Nano Technology, University of Kashan, Kashan , Amooaghaei, R Department of Biology, University of Shahrekord
Abstract :
In the present study, the interaction between nano-TiO2 and
lysozyme was investigated by the method of UV-Vis detection and
fluorescence spectroscopic techniques. The thermal denaturation of
lysozyme has been investigated in the presence and absence of
nano-TiO2 over the temperature range (293-373) K in different
buffer and pH, using temperature scanning spectroscopy. The
presence of nano-TiO2 caused the destabilization of lysozyme
resulting in a decrease in the temperature of unfolding with an
increase in nano-TiO2 concentration.