Author/Authors :
Nourzadeh, Mahdieh Iranian Center for Endodontic Research - Dental Research Center - Dental School, Shahid Beheshti University of Medical Sciences, Tehran , Amini, Arezu Dental School, Zanjan University of Medical Sciences, Zanjan , Fakoor, Farzaneh Department of microbiology - Medical School, Zanjan University of Medical Sciences, Zanjan , Raoof, Maryam Laboratory of Molecular Neuroscience - Neuroscience Research Center - Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman , Sharififar, Fariba Department of Pharmacognosy - School of Pharmacy, Kerman University of Medical Science, Kerman
Abstract :
Introduction: The aim of this study was to evaluate the antimicrobial effect of Eucalyptus
galbie and Myrtus communis L. methanolic extracts, chlorhexidine (CHX) and sodium
hypochlorite (NaOCl) on Enterococcus faecalis (E. faecalis) as the predominant species
isolated from infected root canals. Methods and Materials: One hundred twenty
mandibular premolars were randomly divided into 8 groups: Eucalyptus galbie (E. galbie)
12.5 mg/mL, Myrtus communis L. (M. communis L.) 6.25 mg/mL, 0.2% CHX, %2 CHX,
2.5% NaOCl, 5.25% NaOCl, positive and negative control group. Sampling was
performed using paper points (from the root canal space lumen) and Gates-Glidden
drills (from the dentinal tubules); then colony forming units (CFU) were counted and
analyzed using the Kruskal-Wallis test, followed by Mann Whitney U test. The level of
significance was set at 0.05. Results: All irrigants reduced more than 99% of bacteria in
root canal. In the presence of M. communis L. and E. galbie, the bacterial count in dentin
were significantly more than CHX and NaOCl groups (P<0.05) except 0.2% CHX in 200
μm and 400 μm depths (P>0.05). Conclusion: Although 5.25% NaOCl was the most
effective irrigant, all agents exerted acceptable antimicrobial activity against E. faecalis.
Keywords :
Antibacterial Agent , Eucalyptus , Myrtus , Root Canal Therapy
