Author/Authors :
Ahmadi – Motamayel, Fatemeh Department of Oral Medicine - Dental school, Hamadan University of Medical Scences - Hamadan , Hendi, Sare Hamadan University of Medical Sciences, Hamadan , Alikhani, Mohammad Yusof Department of Microbiology - Medical school, Hamadan University of Medical Sciences, Hamadan , Khamverdi, Zahra Department of Operative Dentistry - Dental school, Hamadan University of Medical Sciences, Hamadan
Abstract :
Objective: Honey has antibacterial activity. The aim of this study was to evaluate the antibacterial activity of honey on Streptococcus mutans and Lactobacillus. Materials and Methods: In this in vitro study, solutions containing 0%, 5%, 10%, 20%, 50% and100%(w/v) of natural Hamadan honey were prepared. Each blood (nutrient) agar plate was then filled with dilutions of the honey. The strains of bacteria were inoculated in blood agar for 24 hours at 37oC and were adjusted according to the McFarland scale (10×10 cfumcl -1). All assays were repeated 10 times for each of the honey concentrations. Data were analyzed by non parametric Chi-Square test. Statistical significance was set at α=0.05.
Results: Significant antibacterial activity was detected for honey on Streptococcus mutans in concentrations more than 20% and on Lactobacillus in 100% concentra-tion (P<0.05).
Conclusion: It seems that antibacterial activity of honey could be used for pre-vention and reduction of dental caries
Keywords :
Honey , Antibacterial Agents , Streptococcus Mutans Lactobacillus , Dental Caries
