Author/Authors :
Ai, Jafar Department of Tissue Engineering - School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran , Shahverdi, Ahmad Reza Department of Pharmaceutical Biotechnology - School of Pharmacy - Tehran University of Medical Sciences, Tehran, Iran , Ebrahimi Barough, Somayeh Department of Biological Science - Faculty of Biology - University of Kharazmi, Tehran, Iran , Mohseni Kouchesfehani, Homa Department of Biological Science - Faculty of Biology - University of Kharazmi, Tehran, Iran , Heidari, Saeed Clinical Proteomics Research Center - Faculty of Paramedical Science - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Roozafzoon, Reza Stem Cell Preparation Unit - Eye Research Center - Farabi Eye Hospital - Tehran University of Medical Sciences, Tehran, Iran , Verdi, Javad Department of Tissue Engineering - School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran , Khoshzaban, Ahad Stem Cell Preparation Unit - Eye Research Center - Farabi Eye Hospital - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Background: Due to increasing clinical demand for adipose tissue, a suitable cell
for reconstructive adipose tissue constructs is needed. In this study, we investigated
the ability of Human Endometrial-derived stem cells (EnSCs) as a new source of
mesenchymal stem cells to differentiate into adipocytes. EnSCs are the abundant and
easy available source with no immunological response, for cell replacement therapy.
Methods: Single-cell suspensions of EnSCs were obtained from endometrial tissues
from 10 women experiencing normal menstrual cycles, and were cultured at clonal
density (10 cells/cm2) or limiting dilution. Endometrial mesenchymal stem cell
markers were examined flow cytometry. These cells were treated with adipogenicinducing
medium for 28 days. The adipogenic differentiation of the EnSC was assessed
by cellular morphology and further confirmed by Oil Red O staining and RTPCR.
The BM-MSC differentiated into adipocytes in the presence of adipogenic
stimuli for 3 weeks.
Results: The flow cytometric analysis showed that the cells were positive for CD90,
CD105, CD146 and were negative for CD31, CD34.We showed that the key adipocytes
marker PPARa was expressed in mRNA level after 28 days post treatment
(PT).
Conclusion: According to our finding, it can be concluded that EnSCs represent a
useful in vitro model for human adipogenesis, and provide opportunities to study the
stages prior to commitment to the adipocyte lineage.
