Author/Authors :
Hajikhani, Bahareh Reproductive Infections Department of Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR, Tehran, Iran , Motallebi, Tayebeh Department of Microbiology - Islamic Azad University Tehran North Branch, Tehran, Iran , Norouzi, Jamileh Department of Microbiology - Islamic Azad University Tehran North Branch, Tehran, Iran , Bahador, Abbas Department of Microbiology - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Bagheri, Rezvan Reproductive Infections Department of Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR, Tehran, Iran , Asgari, Soheila International Campus - Tehran University of Medical Sciences, Kish, Iran , Chamani-Tabriz, Leili Reproductive Infections Department of Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR, Tehran, Iran
Abstract :
Background: Chlamydia trachomatis is the most reported bacterial sexually transmitted
disease, especially among young women worldwide. The aim of this study
was comparison the prevalence of Chlamydia trachomatis infection in woman with
tubal infertility by means of PCR and cell culture techniques.
Methods: Fifty-one women with confirmed TFI were enrolled in this study in (avicenna
infertility Clinic) between January 2010 and January 2011. Cervical swab and
cytobrush specimens were collected from each patient by gynecologists and sent to
laboratory in transport media. Detection of Chlamydia trachomatis in samples was
performed using PCR and bacteria culture in MacCoy cell line. The data were analyzed
by Fisher’s exact test and independent t-test. Statistical significance was established
at a p-value <0.05.
Results: A significant relation was observed between increased the age of first intercourse
and chlamydial infection. Six (11.7%) samples had positive PCR result,
whereas cell culture results were positive in only 2 (3.9%) samples. A significant relation
was also identified between the duration of infertility and infection (p<0.05)
by PCR versus cell culture method.
Conclusion: The results showed that PCR is a rapid method, compared to cell culture
for detecting Chlamydial organism. It also became clear that the age at first intercourse
is important to predict the likelihood of Chlamydia trachomatis.
Keywords :
Cell culture , Chlamydia trachomatis , Cytobrush , PCR , Swab
