Author/Authors :
Hossein Rashidi, Batool Vali-e-Asr Reproductive Health Research Center - Tehran University of Medical Sciences, Tehran, Iran , Chamani-Tabriz, Leili Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR, Tehran, Iran , Haghollahi, Fadieh Vali-e-Asr Reproductive Health Research Center - Tehran University of Medical Sciences, Tehran, Iran , Jeddi-Tehrani, Mahmood Monoclonal Antibody Research Center - Avicenna Research Institute, ACECR, Tehran, Iran , Mehdi Naghizadeh, Mohammad Department of Biostatistics and Epidemiology - Fassa University of Medical Sciences, Fassa, Iran , Shariat, Mamak Maternal-Fetal-Neonatal Health Research Center - Tehran University of Medical Sciences, Tehran, Iran , Mehdi Akhondi, Mohammad Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR, Tehran, Iran , Bagheri, Rezvan Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR, Tehran, Iran , Asgari, Soheila International Campus - Tehran University of Medical Sciences, Kish, Iran , Wylie, Kevan Consultant in sexual Medicine, Sheffield, UK
Abstract :
Background: Nowadays, Chlamydia trachomatis is known as a causative agent of
infertility. Because of, asymptomatic nature of infection, many may suffer from its
lasting complications such as infertility. This study was performed in Tehran during
April 2007 to April 2008 to compare the prevalence of Chlamydia trachomatis infection
in fertile and infertile women using ELISA and PCR methods.
Methods: Overall, 234 infertile and 223 pregnant women, as the fertile group, participated
in this hospital-based case-control study. After completing an informed
consent form and the questionnaire, first catch urine and blood sample were obtained
for PCR and ELISA (IgG, IgM) tests, respectively. Logistic regression analysis was
used to control possible confounding factors, and determine adjusted odds ratio of
infertility due to the infection.
Results: PCR results revealed that 29 (12.4%) of the infertile and 19 (8.5%) of the
fertile women were positive for C. trachomatis infection (p=0.440). IgG was positive
in 21 (9.0%) of the infertile and 11 (5.0%) in the fertile group (p=0.093). IgM assays
identified that 2 (0.9%) of the infertile and 4 (1.8%) of the fertile women were positive
for the micro-organism (p=0.375).
Conclusion: We found no significant differences among fertile and infertile women for Chlamydia trachomatis infection. Nevertheless, molecular techniques which are more sensitive, more specific and non-invasive can be used to detect C. trachomatis infection.
Keywords :
Case control study , Chlamydia trachomatis , Enzyme-linked immunosorbent assay , Infertility , Polymerase chain reaction
