Author/Authors :
Sharifzadeh Baei, M Faculty of Chemical Engineering - Noshirvani University of Technology, Babol , Najafpour, G.D Faculty of Chemical Engineering - Noshirvani University of Technology, Babol , Lasemi, 'Z Faculty of Chemical Engineering - Noshirvani University of Technology, Babol , Tabandeh, F Biotechnology Department - National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran , Younesi, H Department of Environmental Science - Faculty of Natural Resources and Marine Sciences - Tarbiat Modarres University , Issazadeh, H Faculty of Chemical Engineering - Noshirvani University of Technology, Babol , Khodabandeh, M Biotechnology Department - National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran
Abstract :
In the present research, whey was used as useful substrate which retained from permeates of dairy industry. The obtained whey was hydrolyzed to cleave its main carbon source, lactose to glucose and galactose. The hydrolyzed products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Azohydromonas lata DSMZ 1123. The biosynthesis of PHA copolyesters containing 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units from hydrolyzed whey permeate and valerate were investigated. The application of hydrolyzed whey permeate turned out to be advantageous compared to utilization of pure sugars. Therefore, fermentation under controlled conditions (agitation rate of 250 rpm, 18 h incubated inoculum, 72 h fermentation time and temperature set at 30°C) was performed. As a result, maximum amount biopolymer obtained was1.66 g/L. The biopolymer consisted 1.21 and 0.45 g/L of P(3HB) and P(3HV), respectively.
Keywords :
Azohydromonas lata , Cheese whey , Lactose , 3-hydroxybutyrate (3HB) , 3-hydroxyvalerate , (3HV) , Polyhydroxyalkanoate
