Author/Authors :
Sardarabadi, Hadi Department of Biology - Faculty of Science - Ferdowsi University of Mashhad, Mashhad , Mashreghi, Mansour Department of Biology - Faculty of Science - Ferdowsi University of Mashhad, Mashhad , Jamialahmadi, Khadijeh Mashhad University of Medical Sciences, Mashhad , Dianat, Tahere Yazd University, Yazd
Abstract :
Background and Objective: Nanoscopic life forms called Nanobacteria or calcifying nanoparticles (CNP) are unconventional
agents. These novel organisms are very small (0.1 to 0.5 microns) and possess unusual properties such as high resistance to
heat and routine antimicrobial agents. Nanobacteria are 100 times smaller than bacteria and protected by a shell of apatite, so
they could be as candidate for emerging and progress of in vivo pathological calcification. In this study, the inhibitory effect
of broad-spectrum antibiotics on growth of these new forms of life has been investigated.
Material and Methods: Powdered urinary and kidney stones were demineralized with HCl and neutralized with
appropriate buffers and became filtered. Finally suspension was incubated in DMEM medium with Fetal Bovine Serum
(FBS) and broad-spectrum antibiotics (100U/ml for penicillin and 100μg/ml for streptomycin) for 60 days.
Results: In the presence of broad-spectrum antibiotics, Scanning Electron Micrographs (SEM) showed a spherical shape
of these nanobacteria. Also, Energy Dispersive X-ray spectroscopy (EDS) showed a pick for calcium and phosphor.
Transmission Electron Microscopy (TEM) results illustrated cover around the nanobacteria.
Conclusion: The growth of calcifying nanoparticles after adding the broad-spectrum antibiotics may be due to their apatite
hard shells supporting them against penetration of the antibiotics.
