Author/Authors :
khoshkharam-Roodmajani, Hossein Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Sarvari, Jamal Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Bazargani, Abdollah Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Kandekar-Ghahraman, Mohammad Reza Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Nazari-Alam, Ali Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Motamedifar, Mohammad Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz
Abstract :
Background and Objectives: To investigate coagulase gene polymorphisms of MRSA and MSSA isolates from Shiraz
teaching hospitals from 2011 to 2012.
Materials and Methods: A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three
major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates
were subjected to molecular typing on the basis of coagulase enzyme gene polymorphism by PCR-RFLP.
Results: There were 27 and 28 different RFLP patterns for AluI and HaeIII restriction enzymes respectively. This study
showed that the discriminatory power of coagulase gene typing by Hae III enzyme was more than that of Alu I enzyme.
Conclusion: PCR-RFLP method is rapid, reproducible, simple and efficient for typing Staphylococcus aureus isolated from
clinical specimens. This study showed that Hae III discriminatory power is better than Alu I for typing Staphylococcus aureus isolates.
Keywords :
Antibiotic resistance , MRSA , PCR , RFLP , Coagulase gene
