• Title of article

    Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene

  • Author/Authors

    khoshkharam-Roodmajani, Hossein Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Sarvari, Jamal Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Bazargani, Abdollah Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Kandekar-Ghahraman, Mohammad Reza Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Nazari-Alam, Ali Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz , Motamedifar, Mohammad Department of Bacteriology and Virology - Faculty of Medicine - Shiraz HIV/ AIDS Research Center (SHARC) - Shiraz University of Medical Sciences, Shiraz

  • Pages
    7
  • From page
    246
  • To page
    252
  • Abstract
    Background and Objectives: To investigate coagulase gene polymorphisms of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012. Materials and Methods: A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subjected to molecular typing on the basis of coagulase enzyme gene polymorphism by PCR-RFLP. Results: There were 27 and 28 different RFLP patterns for AluI and HaeIII restriction enzymes respectively. This study showed that the discriminatory power of coagulase gene typing by Hae III enzyme was more than that of Alu I enzyme. Conclusion: PCR-RFLP method is rapid, reproducible, simple and efficient for typing Staphylococcus aureus isolated from clinical specimens. This study showed that Hae III discriminatory power is better than Alu I for typing Staphylococcus aureus isolates.
  • Keywords
    Antibiotic resistance , MRSA , PCR , RFLP , Coagulase gene
  • Journal title
    Astroparticle Physics
  • Serial Year
    2014
  • Record number

    2426793

    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=10&DC=2426793