Title of article :
Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii
Author/Authors :
Alibakhshi, Abbas Department of Biotechnology - School of Advanced Technologies in Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Bandehpour, Mojgan Department of Biotechnology - School of Advanced Technologies in Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Kazemi, Bahram Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Background and Objectives: Polytopic antigens are recently applied for replacing crude antigens, for control of infectious
agents. The surface of the Toxoplasma is covered with immunogenic antigens namely surface antigens (SAGs). These antigens
possess several immunogenic epitopes, inducing immune responses.
Materials and Methods: In this study, a DNA construct comprising of sequences encoding epitopes from SAG1, 2 and 3
was designed and cloned into pET28a expression vector and subsequently expressed and purified, using Ni-NTA column.
Results: The SDS-PAGE and Western blotting analysis showed that polytopic genes were successfully expressed and purified.
Conclusion: The surface antigenic protein of T. gondii can be applied in the future epitope-based applications.
Keywords :
Toxoplasma gondii , Polytopic antigen , SAG , Epitope mapping
Journal title :
Astroparticle Physics
