Author/Authors :
Aboutorabi, Roshanak Infertility Laboratory - Beheshti Hospital - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Behzadi, Ehsan School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Sadegh, Mohammad Javad School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Fatehi, Pooriya School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Semsarzadeh, Soroosh School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Zarrin, Yasaman School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Kazemi, Mohammad Department of Genetics - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Rafiee, Laleh Applied Physiology Research Center - Isfahan University of Medical Sciences, Isfahan, Iran , Mostafavi, Fatemeh Sadat Infertility Laboratory - Beheshti Hospital - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran
Abstract :
Background: According to the literature review, polymorphisms of tumor necrosis
factor alpha’s (TNF-α) promoter region are probably the genetic risk factors of recurrent
pregnancy loss. This study has investigated five single nucleotide polymorphisms
in the TNF-α gene’s promoter region to evaluate their relationship with recurrent
pregnancy loss disorder.
Methods: Blood samples were taken from 65 women with recurrent pregnancy loss
(Case group) and 65 healthy women with a history of successful pregnancy (Control
group). Polymerase chain reaction with high resolution melting (PCR-HRM) analysis
was done to determine the promoter region of -308G/A, -850T/C, -238G/A,
-1031T/C and -863A/C TNF-α polymorphisms. The data were assessed using logistic
regression models. P values less than 0.05 were considered statistically significant.
Results: Significant associations were found between recurrent pregnancy loss and -
863C/A (p=0.000), -308G/A (p=0.045), and -238G/A (p=0.034) polymorphisms. TNF-α
polymorphisms of -863C and -238G may be susceptible factors of recurrent pregnancy
loss cases. The -308G polymorphism has an important role in maintaining pregnancy.
Conclusion: The -863C/A and -238G/A TNF-α polymorphisms are possible genetic
risk factors of recurrent pregnancy loss and might be its predictive markers.
