Author/Authors :
Sadeghi, Sepideh Department of Biology and Anatomical Sciences - Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Talebi, Ali Reza Research and Clinical Center for Infertility - Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Shahedi, Abbas Department of Biology and Anatomical Sciences - Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Moein, Mohammad Reza Research and Clinical Center for Infertility - Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Abbasi Sarcheshmeh, Abolghasem Department of Biology and Anatomical Sciences - Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract :
Background: Tamoxifen (TX) is widely used to treat idiopathic infertility in men.
Using TX has been shown to produce sperm in patients with oligospermia and azoospermia
and improve male fertility. The aim of this study was to evaluate the effects
of TX on DNA and chromatin quality in mice regarding the importance of chromatin
quality and sperm DNA at all stages of reproduction.
Methods: 24 male NMRI mice were divided into 3 groups including dose 0.4 mg/
kg/day that received basal diet and TX, dose 0.6 mg/kg/day that received basal diet
and TX, and group 3 that received vehicle for 35 days as the control. After that, epididymal
spermatozoa were analyzed for nuclear DNA quality. One-way ANOVA
was performed with a Tukey test to compare sperm DNA fragmentation at different
times. The p<0.05 was considered significant.
Results: The use of different doses of TX may have detrimental effects on sperm
chromatin protamination and DNA integrity in mice. According to Acridine Orange
(AO) staining, the rate of increased single-stranded DNA damage was observed at
0/6 mg/kg/day TX dose (p<0.05).
Conclusion: The use of different studied TX doses in the animal sample was found
to increase the amount of protamine deficiency and DNA defect in treated mice.
Keywords :
DNA , Mice , Sperm , Tamoxifen
