Author/Authors :
Mahmoudi, Mohammad Jafar Division of Cardiology - Department of Internal Medicine - Faculty of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Harsini, Sara Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Farhadi, Elham Department of Hematology - Faculty of Allied Medical Science - Iran University of Medical Sciences, Tehran, Iran , Hedayat, Mona Division of Immunology - Boston Children's Hospital - Harvard Medical School, Boston, MA, USA , Taghvaei, Mohammad Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Mahmoudi, Maryam Faculty of Nutrition and Dietetics - Tehran University of Medical Sciences, Tehran, Iran , Sadr, Maryam Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Esfahanian, Nilufar Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Nematipour, Ebrahim Tehran Heart Center - Tehran University of Medical Sciences, Tehran, Iran , Nourijelyani, Keramat Department of Epidemiology and Biostatistics - Faculty of Public Health - Tehran University of Medical Sciences, Tehran, Iran , Amirzargar, Ali Akbar Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Rezaei, Nima Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Background: Inflammatory cytokines have been known to be associated with Chronic
Heart Failure (CHF). Given the importance of cytokines in the context of the failing
heart, the prevalence of Interleukin-2 (IL-2) and Interferon-gamma (IFN-γ) polymorphisms
was studied in patients with CHF due to ischemic heart disease in a casecontrol
study.
Methods: Fifty-six Iranian patients with CHF were enrolled in this study as the case
group and compared with 139 healthy subjects, using polymerase chain reaction with
sequence-specific primers method, so as to determine the frequency of alleles, genotypes
and haplotypes of IFN-γ (+874 A/T) and IL-2 (-330 G/T, +166 G/T) SNPs.
Results: The GG genotype at IL-2 -330 in patients with CHF was significantly overrepresented
in comparison with the control group (p=0.013). Such a positive genotypic
association was also observed for IL-2 +166/TT (p=0.022). Meanwhile, the GT genotype
frequency at IL-2 -330/GT in the patient group was significantly lower than the one in
healthy controls (p=0.049). No significant association was detected between the IFN-γ
gene polymorphisms and individuals’ susceptibility to CHF.
Conclusion: Certain genotypes in IL-2 gene were overrepresented in patients with
CHF, which could render individuals more vulnerable to this disease.
