Author/Authors :
Hedayat, Mona Division of Immunology - Boston Children's Hospital - Harvard Medical School, Boston, MA, USA , Mahmoudi, Mohammad Jafar Division of Cardiology - Department of Internal Medicine - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Taghvaei, Mohammad Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Nematipour, Ebrahim Tehran Heart Center - Tehran University of Medical Sciences, Tehran, Iran , Farhadi, Elham Hematology Department - Faculty of Allied Medical Science - Iran University of Medical Sciences, Tehran, Iran , Esfahanian, Nilufar Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Mahmoudi, Maryam School of Nutrition and Dietetics - Tehran University of Medical Sciences, Tehran, Iran , Sadr, Maryam Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Nourijelyani, Keramat Department of Epidemiology and Biostatistics - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , Amirzargar, Ali Akbar Molecular Immunology Research Center - Tehran University of Medical Sciences, Tehran, Iran , Rezaei, Nima Research Center for Immunodeficiencies - Children's Medical Center - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Background: Proinflammatory cytokines have been known to be elevated in patients
with Chronic Heart Failure (CHF). Given the importance of proinflammatory cytokines
in the context of the failing heart, the prevalence of Tumor Necrosis Factor-α
(TNF-α), Interleukin (IL)-6 polymorphisms in patients with CHF was studied due to ischemic
heart disease.
Methods: Forty three patients with ischemic heart failure were enrolled in this study
and compared with 140 healthy individuals. The allele and genotype frequency of
four Single Nucleotide Polymorphisms (SNPs) within the IL-6 (-174, nt565) and TNF-α
(-308, -238) genes were determined, using Polymerase Chain Reaction with Sequence-
Specific Primers (PCR-SSP) assay.
Results: The frequency of the TNF-α (-238) A/A genotype was significantly higher in
patients comparing to controls (p=0.043), while TNF-α G/A genotype at the same position
decreased significantly, in comparison with controls (p=0.018). The most frequent
haplotype for TNF-α was A/A in the patient group in comparison with controls
(p=0.003). There was no significant difference in allele and genotype frequencies of
IL-6 at positions -174 and nt565, and TNF-α at position -308.
Conclusion: Certain alleles, genotypes, and haplotypes in TNF-α, but not IL-6, gene
were overrepresented in patients with ischemic heart failure, which may, in turn, predispose
individuals to this disease.
