Author/Authors :
Gazor, Rouhollah Department of Anatomy and Cell Biology - Faculty of Medicine - Guilan University of Medical Sciences, Rasht, Iran , Eskandari, Mozhgan Department of Anatomy - Ardabil University of Medical Sciences, Ardabil, Iran , Sharafshah, Alireza Cellular and Molecular Research Center - Faculty of Medicine - Guilan University of Medical Sciences, Rasht, Iran , Bahadori, Mohammad Hadi Department of Anatomy and Cell Biology - Faculty of Medicine - Guilan University of Medical Sciences, Rasht, Iran , Golmohammadi, Mohammad Ghasem Department of Anatomy - Ardabil University of Medical Sciences, Ardabil, Iran , Keshavarz, Parvaneh Cellular and Molecular Research Center - Faculty of Medicine - Guilan University of Medical Sciences, Rasht, Iran
Abstract :
Background: Exact mechanisms of fetal harm following vitrification are still unknown.
This study was conducted to evaluate the cryopreservation impact on the expression
of Epidermal Growth Factor Receptor (EGFR) gene in mouse 2-cell and blastocysts.
Methods: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-
cells and blastocysts after 44-46 and 88-89 hr, respectively. These embryos were divided
into two case and control groups. The fresh case group was cryopreserved using
cryotop and warmed after 4 mounts. Normal 2-cells were selected based on their
morphology and their RNA was extracted. Quantitative expression of EGFR gene in
both groups was investigated by applying real time-PCR.
Results: The statistical Real-Time (RT)-PCR analyses performed using SPSS revealed
that the expression level of EGFR gene was diminished in the case group compared to
the control group.
Conclusion: The current study indicated the negative effect of cryopreservation on
expression amount of EGFR gene in 2-cell and blastocyst mouse embryos.
Keywords :
Vitrification , Gene expression , Embryo , Cryopreservation , Blastocyst
