Title of article :
RNA Loading on Nano-Structured Hyperbranched β-Cyclodextrin
Author/Authors :
Hirbod, Sorina Department of Chemistry - Central Tehran Branch Islamic Azad University, Tehran, Iran , Nafisi, Shohreh Department of Chemistry - Central Tehran Branch Islamic Azad University, Tehran, Iran , Maibach, Howard I Department of Dermatology - University of California, San Francisco, CA, USA
Abstract :
Background: β-Cyclodextrin functionalized hyper-branched polyglycerol (HBCD: β-
CD-g-PG), a biocompatible polymer, has recently been proposed for delivery of poorly
water soluble compounds.
Methods: The present study examines the interaction of HBCD with RNA, utilizing a
constant concentration of RNA and different HBCD/RNA ratios of 1/16 to 1/1, at physiological
condition in an aqueous solution. Circular Dichroism (CD), UV-visible, FTIR
spectroscopic methods, zeta potential and Dynamic Light Scattering (DLS) were used
to analyze the particle formation, particle charge, particle size, aggregation, RNA
conformation, binding constant and mode, and the effect of polymer complexation
on RNA stability.
Results: The results indicate that the interaction of RNA with HBCD leads to the formation
of a linear dendritic supramolecule biopolymer with an overall binding constant
of KHBCD/RNA= 1.25 × 103.
Conclusion: The small sized synthesized polymer can be considered as an appropriate
system for preventing RNA aggregation and protecting the gene by host-guest interaction.
Keywords :
Polymer , Particle size , Gene delivery , Aggregation
Journal title :
Astroparticle Physics
