Author/Authors :
Sajjadi, Sharareh Department of Biology - Roudehen Branch, Islamic Azad University, Roudehen, Iran , Keihan, Amir Homayoun Molecular Biology Research Center - Systems Biology and Poisonings Institute - Baqiyatallah University of Medical Sciences, Tehran, Iran , Norouzi, Parviz Center of Excellence in Electrochemistry - Faculty of Chemistry - University of Tehran, Tehran, Iran , Habibi, Mohammad Mahdi Center of Excellence in Electrochemistry - Faculty of Chemistry - University of Tehran, Tehran, Iran , Eskandari, Khadijeh Nanobiotechnology Research Center - Baqiyatallah University of Medical Sciences, Tehran, Iran , Hadizadeh Shirazi, Najmeh Department of Biology - Roudehen Branch, Islamic Azad University, Roudehen, Iran
Abstract :
An amperometric glucose biosensor was developed based on synergistic contributions
of prussian blue (PB) and a bucky gel (BG) consisting of carbon nanotubes (CNTs) and ionic liquid (IL). The PB nanoparticles were first deposited onto the surface of a BG modified glassy carbon (GC) electrode (BG/GC). Then, the Ni2+ ions were electrochemically inserted into the PB lattice to improve its stability at physiological pH. Afterwards, Glucose oxidase (GOx) was immobilized on the BG/GC electrode using a cross-linking method. Amperometric measurements of glucose were performed at −0.05 V vs. Ag/AgCl in 0.05 M phosphate buffer solution at pH 7.4. The glucose biosensor exhibited a sensitivity of 45.03 μA/(mM.cm2)
with a detection limit of 5×10-7 M. The amperometric response was linear in the range of 5×10-7 to 8.3×10−4 M.
Keywords :
Glucose , Biosensore , Nanoparticle , Carbon Nanotube , Ionic Liquid
